寡肽-5和KGF-2是什麼？與FGF-10有什麼聯系？

重組人角質細胞生長因子（rh-KGF）就是FGF-10的全稱，英語的全稱為：sh-polypeptide-10，而寡肽-5則是在中國的簡稱，有時也叫人寡肽-5

品 名：重組人角質細胞生長因子（rh-KGF）

分 子 量：19.2kD

規 格：10μg、100μg、1mg

形式：凍幹粉

保存條件：-20℃保存、3年

介紹： 重組人角質細胞生長因子（rh-KGF）由原核表達系統（E.coli）重組表達制備，由169個氨基酸殘基組成的非糖基化多肽，分子量為19.2kDa。重組人角質細胞生長因子（rh-KGF）從屬于成纖維細胞生長因子家族。rh-KGF基因表達受多種細胞因子調控。rh-KGF與受體KGFR特異性的結合發揮其多種生物學功能：參與組織、器官的發育；參與組織、器官的發育；參與皮膚、胃、腸、腎、膀胱、肺等上皮的損傷修複；減少放、化療所帶來的副作用，具有損傷防護功能。

Keratinocyte growth factor (KGF), which is also called fibroblast growth factor (FGF)-7, belongs to the FGF family. KGF is not commonly produced by human cancer cells, but the KGF receptor (KGFR) is expressed in most cancer cells and particularly highly expressed in well-differentiated types of cancer.

Recently, it has been reported that vascular endothelial growth factor (VEGF)-A expression is induced by KGF in pancreatic cancer cells. VEGF-A is produced by some cancer cells and plays important roles in the angiogenesis and metastasis of cancer cells including those in the colorectum.

In this study, we examined whether recombinant human KGF (rhKGF) induces major angiogenic growth factors including VEGF-A, FGF-2 and hepatocyte growth factor (HGF) in human colorectal cancer cells (HCT-15), which express a high level of KGFR, but a low or negligible level of KGF. rhKGF significantly increased the VEGF-A expression level in a serum-free medium of HCT-15 cells, but FGF-2 and HGF expression levels were too low to detect.

Furthermore, the expression levels of the angiogenic growth factors were evaluated in KGF-transfected HCT-15 cells, which were induced to stably overexpress KGF by KGF gene transfection and mock-transfected cells (Mock).

KGF and VEGF-A expression levels in the cells and the protein concentrations in serum-free medium were significantly higher in KGF-transfected HCT-15 cells than in Mock cells. In contrast, the FGF-2 and HGF mRNA expression levels were not significantly different between KGF-transfected HCT-15 cells and Mock cells and the protein concentrations in serum-free medium of the cells were below the detection level.

These findings suggest that administration of rhKGF and over-expression of endogenous KGF genes in colorectal cancer cells increase VEGF-A production and may relate to angiogenesis in cancer.

使用說明：

1、KGF凍幹粉穩定性一般，凍幹粉溶解後活性可穩定7d内；

2、用于修複傷口或傷疤時，注意不要污染配置好的溶液；

3、根據需要修複的面積計算使用KGF的量，按照每平方分米（約等于人面部面積）使用2000-5000IU（2-5μg）；

4、使用時注意保持無菌不污染産品。