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亞硝酸鹽測定原理
亞硝酸鹽測定原理
更新时间:2024-08-06 05:09:45

  1、亞硝酸鹽測定原理為采用鹽酸萘乙二胺法測定,硝酸鹽采用镉柱還原法測定。

  2、試樣經沉澱蛋白質、除去脂肪後,在弱酸條件下,亞硝酸鹽與對氨基苯磺酸重氮化後,再與鹽酸萘乙二胺偶合形成紫紅色染料,外标法測得亞硝酸鹽含量。采用镉柱将硝酸鹽還原成亞硝酸鹽,測得亞硝酸鹽總量,由測得的亞硝酸鹽總量減去試樣中亞硝酸鹽含量,即得試樣中硝酸鹽含量。

  3、在酸性溶液中,碘酸鉀和碘化鉀作用後析出的遊離碘,将水中的亞硫酸鹽氧化成硫酸鹽,過量的碘與澱粉作用呈現藍色即為終點。

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